Regulatory

Part:BBa_K1381007

Designed by: Stephanie Herman, Gunta Celma, Megha Biradar, Christoffer Andersson and Martin Friberg   Group: iGEM14_Uppsala   (2014-10-02)

J23101-B0034-YenR

This construct was created to characterise BBa_K1381004, the activator YenR. YenR is an activator originated from Yersinia enterocolitica. When YenR binds to the recognition region, BBa_K1381000 (the yenbox), the expression level of the fused promoter is induced. Both YenR and the yenbox are parts in the Yen system, which is a homolougous system to the Lux system. [1]

The BioBricks that were used for assembling this construct was the promoter BBa_J23101 (J23101) from the Anderson promoter library and the activator in the Yen system BBa_K1381004 (B0034-YenR). To read more about the Yen system and for the results from the characterisation experiments, see BBa_K1381004 (B0034-YenR).


References
[1] Ching-Sung Tsai and Stephen C. Winanas, The quorum-hindered transcription factor YenR of Yersinia enterocolitica inhibits pheromone production and promotes motility via a small non-coding RNA, 2011, Molecular Microbiology 80[2], 556-571


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None